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  • Writer's pictureCrystal Filtration

Got (Properly Pasteurized) Milk? Find Out by Measuring Alkaline Phosphatase with the CDR FoodLab®

Alkaline Phosphatase (ALP) in milk

Alkaline phosphatase (ALP) is an enzyme present in raw milk that is inactivated during heat treatment. The temperature needed to inactivate ALP is slightly higher than the temperature needed to destroy pathogenic bacteria. Thus, testing for ALP can determine the effectiveness of the pasteurization process used.

The CDR FoodLab® is an easy-to-use, pre-calibrated, rapid method for testing a number of parameters in milk and dairy products, including ALP. Within 30 minutes you can know the ALP level of milk(s) with just a few simple steps.

How to use the CDR FoodLab® to test dairy
Overview of how the CDR FoodLab® works

The CDR FoodLab® system utilizes pre-filled, ready-to-use reagent cuvettes specific to each test. For the ALP test, the ALP present in the sample induces the hydrolysis of p-nitrophenyl phosphate in an alkaline solution and forms a yellow-colored complex whose intensity, measured at 420 nm, is directly proportional to the concentration of ALP in the sample.

p-Nitrophenyl phosphate and ALP
Reaction that occurs with reagent kit to measure ALP with the CDR FoodLab®

CDR FoodLab® ALP calibration curve
ALP calibration curve

The pre-calibrated test curve for ALP was developed using various additions of raw milk in pasteurized milk. The values reported are correlated with those reported in the literature (“ALP testing for milk Pasteurization” Cornell University – Dairy science facts 1998).

Measuring range and repeatability of ALP on the CDR FoodLab®
Measuring range and repeatability of ALP on the CDR FoodLab®

In addition to ALP, the CDR FoodLab® can test a number of other parameters in milk and dairy products such as: lactose, milk urea nitrogen (MUN), ammonia, and more. You can find our analyzer options and tests available in our Buyer’s Guide.

Want to make sure your milk is properly pasteurized with the CDR FoodLab®? Contact Us!

Please visit us at the World Dairy Expo in Madison, Wisconsin on October 2nd-6th, Booth Number #578. For more information or to register ➝

World Dairy Expo


2146 Avon Industrial Dr.

Rochester Hills, MI 48309


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